| 质粒名称: | mARG1 cassette 3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 134345 |
| 存储实验室: | Mikhail Shapiro |
|
载体骨架基本信息 |
|
| 载体名称: | pAF PB |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 9002 bp |
| 载体类型: | 哺乳动物表达，合成生物学 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mARG 1 cassete 3,Mammalian acoustic reporter gene1 cassette 3 |
| 物种来源: | Bacillus megaterium |
| 插入大小: | 2721 bp |
| 启动子 | TRE3G |
| 克隆方法: | Gibson克隆 |
| 5' 测序引物 | SP6 Promoter |
| 3' 测序引物 | GAGCCTCTACGACTCCGTGAA |
|
文献引用方法 |
|
| 材料和方法部分: | mARG1 cassette 3 was a gift from Mikhail Shapiro (Addgene plasmid # 134345 ; http://n2t.net/addgene:134345 ; RRID:Addgene_134345) |
| 参考文献部分: | Ultrasound imaging of gene expression in mammalian cells. Farhadi A, Ho GH, Sawyer DP, Bourdeau RW, Shapiro MG. Science. 2019 Sep 27;365(6460):1469-1475. doi: 10.1126/science.aax4804. 10.1126/science.aax4804 PubMed 31604277 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mARG1 cassette 3 9002 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac
trasposon plasmid..
ACCESSION .
VERSION .
KEYWORDS mARG1 cassette 3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9002)
AUTHORS Farhadi A, Ho GH, Sawyer DP, Bourdeau RW, Shapiro MG
TITLE Ultrasound imaging of gene expression in mammalian cells.
JOURNAL Science. 2019 Sep 27;365(6460):1469-1475. doi:
10.1126/science.aax4804.
PUBMED 31604277
REFERENCE 2 (bases 1 to 9002)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9002
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..17
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
repeat_region 147..209
/label=piggyBac right (3') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
polyA_signal 664..745
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(1635..1653)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(1636..1653)
/label=SP6
/note="SP6 promoter, forward primer"
promoter 1726..2075
/label=TRE3GV promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, optimized for
retroviral and lentiviral vectors"
protein_bind 1734..1752
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1770..1788
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1806..1824
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1842..1860
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1878..1896
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1914..1932
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1950..1968
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
regulatory 2089..2098
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2413..2469
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
regulatory 2596..2605
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 2600..2609
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3253..3309
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3592..3648
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4474..4530
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
polyA_signal 4845..4966
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 4936..4955
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
repeat_region 5729..5763
/label=piggyBac left (5') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
primer_bind complement(6254..6270)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6254..6270)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6267..6289)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6278..6294
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6302..6332)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6347..6368
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6485..6502)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6656..7244)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6736..6755)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7415..8275)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8038..8057
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8276..8380)
/gene="bla"
/label=AmpR promoter
rep_origin complement(8406..8861)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8543..8564)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8755..8774
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8988..8
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9002..17
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
ORIGIN
1 gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg
61 ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca
121 cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta
181 aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt
241 tattaatttg aatagatatt aagttttatt atatttacac ttacatacta ataataaatt
301 caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct
361 tctataaagt aacaaaactt ttatgaggga cagccccccc ccaaagcccc cagggatgta
421 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
481 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
541 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
601 ggggatacgg ggaaaaggcc tccacggcca ctagttttcc ccgaaaagtg ccacctgacg
661 tcggcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
721 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
781 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
841 tgattatgat cctctagaca tatgctgcag tcacttgtac agctcatcca tgcccagggt
901 gatgccagcg gcggtccgaa attccagcag caccatgtgg tcccgcttct cgttggggtc
961 cttgctcagc acgctctggg tgctcaggta gtggctatca ggcagcagca cggggccatc
1021 tccgatgggg gtgttctgct ggtagtggtc ggccagctgc acgctgccat cttccacgtt
1081 gtgccggatc ttgaagttca ctttgatgcc gtttttctgc ttcacggcca tgatgtagat
1141 gttgtggctg ttgaagttgt actccagctt gtggcccagg atgttgccgt cctctttgaa
1201 gtccacgccc ttcagctcga tccggttcac gagggtgtcg ccctcgaact tcacttcggc
1261 tctggtcttg taggtgccgt cgtccttgaa gaagatggtc cgttcctgca cgtagccctc
1321 gggcatggcg ctcttgaaga aatcgtgctg cttcatgtgg tcggggtatc tggcgaagca
1381 ctgcacgccg tgagacagtg tggtcacgag ggtaggccaa ggcacgggca gcttgccggt
1441 ggtgcagatg aacttcaggg tcagcttgcc atttgtggcg tcgccttcgc cctctccccg
1501 cacagagaac ttgtggccgt tcacgtcgcc atccagttcc accaggatgg gcaccacgcc
1561 ggtgaacagt tcctcgccct tggacaccat ggtgaagggt actggatccg agctcggtac
1621 ctgcaggcgt accttctata gtgtcaccta aatgcgatct gacggttcac taaacgagct
1681 ctgcttatat aggcctccca ccgtacacgc cacctcgaca tactcgagtt tactccctat
1741 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
1801 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
1861 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
1921 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
1981 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
2041 ctggagcaat tccacaacac ttttgtctta tacttggtac ctatgcatgc caccatggcc
2101 gtggaacaca acatgcagag cagcaccatc gtggacgtgc tggaaaagat cctggacaag
2161 ggcgtcgtga tcgccgggga catcacagtg ggaatcgccg acgtggaact gctgaccatc
2221 aagatccggc tgatcgtggc cagcgtggac aaggccaaag aaatcggcat ggattggtgg
2281 gagaacgacc cctacctgag cagcaagggc gccaacaaca aggccctgga agaggaaaac
2341 aagatgctgc acgagcggct gaaaacactg gaagagaaga tcgagacaaa gcgcggtgcc
2401 ccgggatctg gcgcaacaaa ttttagtctt ttaaagcagg caggagacgt cgaggaaaac
2461 cctggacccg tgagcgagac aaacgagaca ggcatctaca tcttcagcgc catccagaca
2521 gacaaggatg aggaattcgg cgccgtggaa gtggaaggga ccaaggctga gacattcctg
2581 atccggtata aggacgccgc catggtggcc gccgaagtgc ccatgaagat ctaccacccc
2641 aaccggcaga acctgctgat gcaccagaat gccgtggccg ccatcatgga caagaacgac
2701 accgtgatcc ccatcagctt cggcaacgtg ttcaagagca aagaggacgt gaaggtgctc
2761 ctggaaaacc tgtaccccca gttcgagaag ctgttccccg ccatcaaggg aaagatcgaa
2821 gtgggcctga aggtgatcgg caagaaagag tggctcgaaa agaaagtgaa cgagaacccc
2881 gagctggaaa aagtgtccgc cagcgtgaag ggcaagagcg aggccgctgg ctactacgag
2941 agaatccagc tgggcggcat ggcccagaag atgttcacaa gcctgcagaa agaagtgaaa
3001 accgacgtgt tcagccccct ggaagaagcc gccgaggccg ccaaagccaa tgagcctaca
3061 ggcgaaacaa tgctgctgaa cgccagcttc ctgatcaaca gagaggatga ggccaagttc
3121 gacgagaaag tcaatgaggc ccacgagaac tggaaggata aggccgactt ccactacagc
3181 ggcccctggc ccgcctacaa cttcgtgaac atccggctga aggtggaaga gaagggggca
3241 cctggctcgg gagcgaccaa cttctcatta ctcaaacaag ccggagacgt tgaggagaat
3301 ccaggccctg tgctgcacaa gctcgtgacc gcccccatca acctggtcgt gaagatcggc
3361 gagaaggtgc aggaagaggc cgacaagcag ctgtacgacc tgcccaccat ccagcagaag
3421 ctgatccagc tgcagatgat gttcgagctg ggcgagatcc ccgaggaagc cttccaggaa
3481 aaagaggacg aactgctgat gagatacgag atcgccaagc ggcgcgagat tgagcagtgg
3541 gaagaactga cccagaagcg gaatgaggaa agcggtgccc cgggatctgg cgcaacaaat
3601 tttagtcttt taaagcaggc aggagacgtc gaggaaaacc ctggacccgt gggcgagctg
3661 ctgtacctct acggcctgat ccccaccaaa gaggccgctg ctatcgagcc cttcccattc
3721 tacaagggct tcgacggcga gcacagcctg taccctatcg ccttcgacca agtgaccgcc
3781 gtggtgttca agctggacgc cgacacctac agcgagaaag tgatccagga aaagatggaa
3841 caggacatga gctggctgca ggaaaaggcc ttccaccacc acgagacagt ggccgccctg
3901 tatgaggaat tcaccatcat ccccctgaag ttctgcacca tctataaggg agaggaatcc
3961 ctgcaggccg ccatcgagat caacaaagag aagatcgaaa actccctgac cctgctgcag
4021 ggcaacgagg aatggaacgt gaagatctac tgcgacgaca ccgagctgaa gaagggcatc
4081 agcgagacaa acgagagcgt gaaggccaag aagcaggaaa tcagccacct gagccccggc
4141 agacagttct tcgagaagaa gaagattgac cagctcatcg agaaagagct ggaactgcac
4201 aagaacaaag tgtgcgagga aatccacgac aagctgattg agctgagcct ctacgactcc
4261 gtgaagaaga actggtccaa ggacgtgaca ggcgctgccg aacagatggc ctggaacagc
4321 gtgttcctgc tgcccagcct gcagatcacc aagttcgtga acgagatcga ggaactccag
4381 cagcggctgg agaacaaggg atggaagttc gaagtgaccg gcccctggcc tccctaccac
4441 ttcagcagct ttgccggggc acctggctcg ggagcgacca acttctcatt actcaaacaa
4501 gccggagacg ttgaggagaa tccaggccct gtgcagcccg tgtcccaggc caacggcaga
4561 atccacctgg atcccgatca ggccgaacag ggactggccc agctcgtgat gaccgtgatc
4621 gagctgctgc ggcagatcgt ggaacggcac gccatgagaa gagtggaagg cggcaccctg
4681 accgacgagc agatcgagaa tctgggaatc gccctgatga acctggaaga gaagatggac
4741 gagctgaaag aggtgttcgg actggacgcc gaggacctga acatcgacct gggccctctg
4801 ggcagcctgc tgtgaactag ttcgataccg tcgaccgtta actaaacttg tttattgcag
4861 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
4921 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt
4981 gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc cgggggacaa
5041 gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc
5101 tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca
5161 gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg
5221 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
5281 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
5341 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
5401 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc
5461 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
5521 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga
5581 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca
5641 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt
5701 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta
5761 gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc
5821 ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt
5881 gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa
5941 cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg
6001 ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg
6061 gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc
6121 ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat
6181 cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct
6241 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
6301 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
6361 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
6421 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
6481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
6541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
6601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
6661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
6721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
6781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
6841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
6901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
6961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
7021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
7081 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
7141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
7201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
7261 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
7321 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
7381 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
7441 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
7501 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
7561 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
7621 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
7681 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
7741 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
7801 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
7861 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
7921 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
7981 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
8041 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
8101 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
8161 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
8221 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
8281 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
8341 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
8401 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
8461 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
8521 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
8581 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
8641 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
8701 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
8761 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
8821 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
8881 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
8941 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
9001 tt
//
